ASTM E1657 : 98(2006)

Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography

Standard Details

1.1 This practice covers the testing of the performance of a variable-wavelength photometric detector (VWPD) used as the detection component of a liquid-chromatographic (LC) system operating at one or more wavelengths in the range 190 to 800 nm. Many of the measurements are made at 254 nm for consistency with Practice E 685. Measurements at other wavelengths are optional.

1.2 This practice is intended to describe the performance of the detector both independently of the chromatographic system (static conditions) and with flowing solvent (dynamic conditions).

1.3 For general liquid chromatographic procedures, consult Refs (1-9).

1.4 For general information concerning the principles, construction, operation, and evaluation of liquid-chromatography detectors, see Refs (10, 11)in addition to the sections devoted to detectors in Refs (1-7).

1.5 The values stated in SI units are to be regarded as standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.