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Biotechnology — Massively parallel sequencing — Part 3: General requirements and guidance for metagenomics
This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:
a) sampling strategy and process, including type, storage, transportation, extraction, quality;
b) nucleic acid library preparation
c) design and review process including sequencing strategy and assessment;
d) database construction;
e) bioinformatics analysis and report
f) validation and verification for bioinformatics pipeline, and database
This document applies to laboratories and research organizations.
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