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ISO
ISO 5510:1984

ISO 5510:1984

Animal feeding stuffs — Determination of available lysine

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General Information

Status :

Active

Standard Type : Main

Document No : ISO 5510:1984

Document Year : 1984

Pages : 5

Edition : 1

Section Volume : Animal feeding stuffs (ISO/TC 34/SC 10.)

ICS : Animal feeding stuffs (65.120)

The method consists in hydrolysing with hydrochloric acid of a ground test portion, dollowed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm. Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.